解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The multi-domain splicing factor RBM5 regulates the balance between antagonistic isoforms of the apoptosis-control genes FAS/CD95, Caspase-2 and AID. An OCRE (OCtamer REpeat of aromatic residues) domain found in RBM5 is important for alternative splicing regulation and mediates interactions with components of the U4/U...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14707
更新日期:2016-11-29 00:00:00
abstract::Hepcidin is the master regulator of systemic iron homeostasis. Derived primarily from the liver, it inhibits the iron exporter ferroportin in the gut and spleen, the sites of iron absorption and recycling respectively. Recently, we demonstrated that ferroportin is also found in cardiomyocytes, and that its cardiac-spe...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19804
更新日期:2016-11-29 00:00:00
abstract::Polypyrimidine-tract binding protein PTBP1 can repress splicing during the exon definition phase of spliceosome assembly, but the assembly steps leading to an exon definition complex (EDC) and how PTBP1 might modulate them are not clear. We found that PTBP1 binding in the flanking introns allowed normal U2AF and U1 sn...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19743
更新日期:2016-11-24 00:00:00
abstract::Selective autophagy is mediated by cargo receptors that link the cargo to the isolation membrane via interactions with Atg8 proteins. Atg8 proteins are localized to the membrane in an ubiquitin-like conjugation reaction, but how this conjugation is coupled to the presence of the cargo is unclear. Here we show that the...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18544
更新日期:2016-11-23 00:00:00
abstract::Mammalian lifespan differs by >100 fold, but the mechanisms associated with such longevity differences are not understood. Here, we conducted a study on primary skin fibroblasts isolated from 16 species of mammals and maintained under identical cell culture conditions. We developed a pipeline for obtaining species-spe...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19130
更新日期:2016-11-22 00:00:00
abstract::The basal ganglia are known to be involved in the planning, execution and control of gripping force and movement vigour. Here we aim to define the nature of the basal ganglia control signal for force and to decode gripping force based on local field potential (LFP) activities recorded from the subthalamic nucleus (STN...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19089
更新日期:2016-11-18 00:00:00
abstract::The link between the combined action of neuromodulators in the brain and global brain states remains a mystery. In this study, using biophysically realistic models of the thalamocortical network, we identified the critical intrinsic and synaptic mechanisms, associated with the putative action of acetylcholine (ACh), G...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18607
更新日期:2016-11-16 00:00:00
abstract::HIV-1 infection cannot be cured because the virus persists as integrated proviral DNA in long-lived cells despite years of suppressive antiretroviral therapy (ART). In a previous paper (Zanini et al, 2015) we documented HIV-1 evolution in 10 untreated patients. Here we characterize establishment, turnover, and evoluti...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18889
更新日期:2016-11-15 00:00:00
abstract::Neural circuits that control copulation in male flies have been identified. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.22215
更新日期:2016-11-15 00:00:00
abstract::Neuroligins (NLGNs) are postsynaptic cell adhesion molecules that interact trans-synaptically with neurexins to mediate synapse development and function. NLGN2 is only at inhibitory synapses while NLGN3 is at both excitatory and inhibitory synapses. We found that NLGN3 function at inhibitory synapses in rat CA1 depend...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19236
更新日期:2016-11-02 00:00:00
abstract::bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Fu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17537
更新日期:2016-10-28 00:00:00
abstract::Mechanical force and Wnt signaling activate β-catenin-mediated transcription to promote proliferation and tissue expansion. However, it is unknown whether mechanical force and Wnt signaling act independently or synergize to activate β-catenin signaling and cell division. We show that mechanical strain induced Src-depe...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19799
更新日期:2016-10-26 00:00:00
abstract::Evolution persistently undermines vector control programs through insecticide resistance. Here we propose a novel strategy which instead exploits evolution to generate and sustain new control tools. Effective spatial repellents are needed to keep vectors out of houses. Our approach generates such new repellents by com...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15416
更新日期:2016-10-25 00:00:00
abstract::A small transparent crustacean called Parhyale hawaiensis has become a powerful model system for the study of limb and appendage regeneration. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.21583
更新日期:2016-10-25 00:00:00
abstract::Translesion DNA synthesis (TLS) during S-phase uses specialized TLS DNA polymerases to replicate a DNA lesion, allowing stringent DNA synthesis to resume beyond the offending damage. Human TLS involves the conjugation of ubiquitin to PCNA clamps encircling damaged DNA and the role of this post-translational modificati...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19788
更新日期:2016-10-22 00:00:00
abstract::Trypanosome parasites are hiding in human skin, a discovery that may undermine efforts to eliminate sleeping sickness by 2020. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.21506
更新日期:2016-10-14 00:00:00
abstract::Temporary circuits amplify spontaneous activity in the visual system of neonatal rats. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.21236
更新日期:2016-10-11 00:00:00
abstract::Notch signaling plays a crucial role in controling the proliferation and differentiation of stem and progenitor cells during embryogenesis or organogenesis, but its regulation is incompletely understood. BLOS2, encoded by the Bloc1s2 gene, is a shared subunit of two lysosomal trafficking complexes, biogenesis of lysos...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18108
更新日期:2016-10-10 00:00:00
abstract::C-type inactivation of potassium channels fine-tunes the electrical signaling in excitable cells through an internal timing mechanism that is mediated by a hydrogen bond network in the channels' selectively filter. Previously, we used nonsense suppression to highlight the role of the conserved Trp434-Asp447 indole hyd...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18976
更新日期:2016-10-06 00:00:00
abstract::In response to cell death signals, an active apoptosome is assembled from Apaf-1 and procaspase-9 (pc-9). Here we report a near atomic structure of the active human apoptosome determined by cryo-electron microscopy. The resulting model gives insights into cytochrome c binding, nucleotide exchange and conformational ch...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17755
更新日期:2016-10-04 00:00:00
abstract::Viral replicase recruitment and long-range RNA interactions are essential for RNA virus replication, yet the mechanism of their interplay remains elusive. Flaviviruses include numerous important human pathogens, e.g., dengue virus (DENV) and Zika virus (ZIKV). Here, we revealed a highly conserved, conformation-tunable...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17636
更新日期:2016-10-01 00:00:00
abstract::Corticostriatal afferents can engage parvalbumin-expressing (PV+) interneurons to rapidly curtail the activity of striatal projection neurons (SPNs), thus shaping striatal output. Schemes of basal ganglia circuit dynamics generally consider striatal PV+ interneurons to be homogenous, despite considerable heterogeneity...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.16088
更新日期:2016-09-26 00:00:00
abstract::LAMP1 and LAMP2 proteins are highly abundant, ubiquitous, mammalian proteins that line the lysosome limiting membrane, and protect it from lysosomal hydrolase action. LAMP2 deficiency causes Danon's disease, an X-linked hypertrophic cardiomyopathy. LAMP2 is needed for chaperone-mediated autophagy, and its expression i...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21635
更新日期:2016-09-24 00:00:00
abstract::Understanding how the brain captures transient experience and converts it into long lasting changes in neural circuits requires the identification and investigation of the specific ensembles of neurons that are responsible for the encoding of each experience. We have developed a Robust Activity Marking (RAM) system th...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.13918
更新日期:2016-09-23 00:00:00
abstract::Despite their importance in sexual differentiation and reproduction, Y chromosome genes are rarely described because they reside in repeat-rich regions that are difficult to study. Here, we show that Guy1, a unique Y chromosome gene of a major urban malaria mosquito Anopheles stephensi, confers 100% female lethality w...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19281
更新日期:2016-09-20 00:00:00
abstract::Congenital diaphragmatic hernia (CDH) is a severe birth defect. Wt1-null mouse embryos develop CDH but the mechanisms regulated by WT1 are unknown. We have generated a murine model with conditional deletion of WT1 in the lateral plate mesoderm, using the G2 enhancer of the Gata4 gene as a driver. 80% of G2-Gata4(Cre);...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.16009
更新日期:2016-09-19 00:00:00
abstract::Previous studies had shown that the integration of genome wide expression profiles, in metabolic tissues, with genetic and phenotypic variance, provided valuable insight into the underlying molecular mechanisms. We used RNA-Seq to characterize hypothalamic transcriptome in 99 inbred strains of mice from the Hybrid Mou...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15614
更新日期:2016-09-13 00:00:00
abstract::Evolutionary changes in transcription networks are an important source of diversity across species, yet the quantitative consequences of network evolution have rarely been studied. Here we consider the transcriptional 'rewiring' of the three GAL genes that encode the enzymes needed for cells to convert galactose to gl...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18981
更新日期:2016-09-10 00:00:00
abstract::Claims that magnetic fields can be used to manipulate biological systems contradict some basic laws of physics. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.19569
更新日期:2016-09-08 00:00:00
abstract::One of the major unanswered questions in evolutionary biology is when and how the transition between diderm (two membranes) and monoderm (one membrane) cell envelopes occurred in Bacteria. The Negativicutes and the Halanaerobiales belong to the classically monoderm Firmicutes, but possess outer membranes with lipopoly...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14589
更新日期:2016-08-31 00:00:00
abstract::Ki-67 and RepoMan have key roles during mitotic exit. Previously, we showed that Ki-67 organizes the mitotic chromosome periphery and recruits protein phosphatase 1 (PP1) to chromatin at anaphase onset, in a similar manner as RepoMan (Booth et al., 2014). Here we show how Ki-67 and RepoMan form mitotic exit phosphatas...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.16539
更新日期:2016-08-30 00:00:00
abstract::Brief periods of sleep loss have long-lasting consequences such as impaired memory consolidation. Structural changes in synaptic connectivity have been proposed as a substrate of memory storage. Here, we examine the impact of brief periods of sleep deprivation on dendritic structure. In mice, we find that five hours o...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.13424
更新日期:2016-08-23 00:00:00
abstract::Smoothened (Smo) inhibition by Patched (Ptch) is central to Hedgehog (Hh) signaling. Ptch, a proton driven antiporter, is required for Smo inhibition via an unknown mechanism. Hh ligand binding to Ptch reverses this inhibition and activated Smo initiates the Hh response. To determine whether Ptch inhibits Smo strictly...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17634
更新日期:2016-08-23 00:00:00
abstract::A new study reveals that a protein called talin forms a vital link between microtubules and focal adhesions at the surface of cells. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.19733
更新日期:2016-08-23 00:00:00
abstract::Altered insulin signaling has been linked to widespread nervous system dysfunction including cognitive dysfunction, neuropathy and susceptibility to neurodegenerative disease. However, knowledge of the cellular mechanisms underlying the effects of insulin on neuronal function is incomplete. Here, we show that cell aut...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.16807
更新日期:2016-08-15 00:00:00
abstract::Nervous systems distinguish between self- and other-generated movements by monitoring discrepancies between planned and performed actions. To do so, corollary discharges are conveyed to sensory areas and gate expected reafference. Such gating is observed in neonatal rats during wake-related movements. In contrast, twi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18749
更新日期:2016-08-03 00:00:00
abstract::Interactions between membrane protein interfaces in lipid bilayers play an important role in membrane protein folding but quantification of the strength of these interactions has been challenging. Studying dimerization of ClC-type transporters offers a new approach to the problem, as individual subunits adopt a stable...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17438
更新日期:2016-08-03 00:00:00
abstract::Although it is clear that trisomy 21 causes Down syndrome, the molecular events acting downstream of the trisomy remain ill defined. Using complementary genomics analyses, we identified the interferon pathway as the major signaling cascade consistently activated by trisomy 21 in human cells. Transcriptome analysis rev...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.16220
更新日期:2016-07-29 00:00:00
abstract::Newly born cells either continue to proliferate or exit the cell division cycle. This decision involves delaying expression of Cyclin E that promotes DNA replication. ORC1, the Origin Recognition Complex (ORC) large subunit, is inherited into newly born cells after it binds to condensing chromosomes during the precedi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12785
更新日期:2016-07-26 00:00:00
abstract::The Fluc family of F(-) ion channels protects prokaryotes and lower eukaryotes from the toxicity of environmental F(-). In bacteria, these channels are built as dual-topology dimers whereby the two subunits assemble in antiparallel transmembrane orientation. Recent crystal structures suggested that Fluc channels conta...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18767
更新日期:2016-07-23 00:00:00